Figure 4.

HIF-1α silencing does not impact 53BP1 levels of foci. (a) Kinetics of 53BP1 foci after X-rays and C-ions ± siHIF-1α. The percentages of foci per nucleus were calculated for each condition considering the X-ray normoxic peak as the reference (means ± SD). (b,c) represent respectively the 53BP1 peak-induction from the basal levels and the decay-rate from the peak to 6 h, as determined from the data shown in (a). (d) Residual foci were quantified at 24 h and each condition was compared to their respective normoxic condition (two-way ANOVA test, ns = non-significant). (n = 3 photons; n = 2 C-ions).