Figure 4.

CHK1i+LDHU enhances adaptive and innate immune responses in vivo. (A) YUMMUV1.7 mouse melanoma line was established in immune-competent C57BL/6J mice (5 mice/group). When tumours had reached 200–300 mm³ they were treated three times/week every other day with 50 mg/kg SRA737 + 100 mg/kg HU by oral gavage for three weeks. Tumour volume was measured using callipers. The blue arrowheads indicate the treatment days. (B–H) Three tumours for each treatment were harvested 2 days after the final treatment and analysed using NanoString Mouse PanCancer Immune Profiling Panel. Heat maps of the changes in expression compared to vehicle-treated control of significantly altered genes are shown. The transcripts were partitioned into cell types and processes they are associated with.