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. 2021 Jul 26;13(15):3752. doi: 10.3390/cancers13153752

Figure 6.

Figure 6

TCF7L2 binds the rs10514231 region and regulates the ATP6AP1L gene expression. (a) ChIP-qPCR analysis for TCF7L2 chromatin binding at the rs10514231 region. Input DNA and IgG ChIP DNA were used as the negative control. Values are the means ± SD; * p < 0.05, *** p < 0.001, two-tailed Student’s t-test. (b) Sanger sequencing chromatogram with the PCR product of ChIP DNA and input DNA. (c) The under-peak area of C and T alleles in (b) was quantified by EditR 1.0.9 online software. (d) RT-qPCR analysis of ATP6AP1L expression in MDA-MB-453 cells with lentiviral TCF7L2 shRNA knockdown. Values are the means ± SD; ** p < 0.01, *** p < 0.001, two-tailed Student’s t-test. (e) The mRNA expression level of the ATP6AP1L gene in MDA-MB-453 cells upon lentiviral overexpression of the TCF7L2 gene. Values are the means ± SD; ** p < 0.01, *** p < 0.001, two-tailed Student’s t-test.