Table 2.
5,6 α-EC (µg/mL) | 5,6 β-EC (µg/mL) | CI-24 h | CI-48 h | Effect |
---|---|---|---|---|
12 * | 20 * | 0.18 ± 0.03 | 0.22 ± 0.2 | Synergistic |
26 ** | 27 ** | 8.52−4 ± 0.13 | 9.09−4 ± 0.24 | Synergistic |
U266 cell lines were seeded in 24-well plates at the density of 2 × 105 cells per well, then treated for 24 h or 48 h the with the indicated concentrations of 5,6 α-EC or 5,6 β-EC corresponding to the IC25 * and IC50 **. Cell viability was assessed using an MTT assay. The CI, presented as means ± SD, were calculated with the CompuSyn software (www.combosyn.com/, accessed on 21 July 2021).