Figure 3.

Effect of EWSR1–FLI1 gene editing on cell proliferation. (a) A673/Cas9/sgRNA cells were continuously maintained in culture and the cumulative population doubling was recorded. The A673/TR/shEF cells were also continuously maintained in culture in the absence or presence of doxycycline (1 µg/mL). (b) Quantification of the population doubling time in each cycle of cell seeding–trypsinization. Mean ± SD of three independent experiments is shown (ns, not significant; ** p < 0.01, *** p < 0.001; two-way ANOVA Tukey’s post hoc vs. control). (c) The cell cycle was analyzed by flow cytometry in non-synchronized cells. The percentages of cells in each cell cycle phase are indicated. One representative experiment out of two independent experiments performed with similar results is shown.