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. 2021 Jul 31;13(15):3869. doi: 10.3390/cancers13153869

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of the K16 expression in EMT-induced cells. (A) Microscopic images showed morphological changes in MCF7 cells during EMT induction; scale bar represents 100 μm. (B) Heat-map of EMT/MET specific genes expression in EMT-induced cells at different culture conditions and time points; relative mRNA expression values were normalized to GAPDH and subsequently displayed relative to gene expression as a fold change 2^{−(∆∆CT)}. (C) Protein expression profile of K16, CDH1, VIM, and SNAI2 during EMT induction on MCF7 cells. HSC70 was used as a reference control. The relative ratio of protein expression of (D) CDH1; (E) K16; (F) SNAI2.