Table 1.
Specific primers used in RT-qPCR.
| Gene | Forward Primer (5’-3′) | Reverse Primer (5’-3′) | Product Length |
|---|---|---|---|
| E-cadherin (CDH1) | CGAGAGCTACACGTTCACGG | GGGTGTCGAGGGAAAAATAGG | 119 bp |
| N-cadherin (CDH2) | TGCGGTACAGTGTAACTGGG | GAAACCGGGCTATCTGCTCG | 123 bp |
| Vimentin (VIM) | GACGCCATCAACACCGAGTT | CTTTGTCGTTGGTTAGCTGGT | 238 bp |
| SNAI2 (SLUG) | TGTGACAAGGAATATGTGAGCC | TGAGCCCTCAGATTTGACCTG | 203 bp |
| SNAI1 | ACTGCAACAAGGAATACCTCAG | GCACTGGTACTTCTTGACATCTG | 242 bp |
| ZEB1 | GATGATGAATGCGAGTCAGATGC | ACAGCAGTGTCTTGTTGTTGT | 86 bp |
| ZEB2 | GGAGACGAGTCCAGCTAGTGT | CCACTCCACCCTCCCTTATTTC | 107 bp |
| TWIST1 | AAGGCATCACTATGGACTTTCTCT | GCCAGTTTGATCCCAGTATTTT | 96 bp |
| WNT5A | ATTCTTGGTGGTCGCTAGGTA | CGCCTTCTCCGATGTACTGC | 159 bp |
| NOTCH1 | GAGGCGTGGCAGACTATGC | CTTGTACTCCGTCAGCGTGA | 140 bp |
| KRT8 | CAGAAGTCCTACAAGGTGTCCA | CTCTGGTTGACCGTAACTGCG | 194 bp |
| KRT18 | GCTCAGATCTTCGCAAATACTGT | CTTCCTCTTCGTGGTTCTTCTTC | 250 bp |
| KRT19 | ACCAAGTTTGAGACGGAACAG | CCCTCAGCGTACTGATTTCCT | 181 bp |
| KRT16 | GACCGGCGGAGATGTGAAC | CTGCTCGTACTGGTCACGC | 91 bp |
| CD24 | CTCCTACCCACGCAGATTTATTC | AGAGTGAGACCACGAAGAGAC | 166 bp |
| CD44 | CTGCCGCTTTGCAGGTGTA | CATTGTGGGCAAGGTGCTATT | 109 bp |
| GAPDH | GGAGCGAGATCCCTCCAAAAT | GGCTGTTGTCATACTTCTCATGG | 197 bp |