Fig 4. Low DENV blocker mosquitoes have a higher predicted deleterious mutation load than High DENV blockers; an effect primarily driven by higher allele frequency of potential deleterious SNPs on chromosome 1 in the Low blocking line.

Boxplots represent screened wMel-infected A. aegypti mosquitoes from WMPB selected lines for differences in (A) predicted mean deleterious load, (B) mean allele frequency of potential deleterious SNPs and (C) mean inverted SIFT score, as a proxy for accumulation of more severe SNPs across groups (High: dark blue—Low: yellow) and chromosomes (red). Significant comparisons expressed via P-values were determined via Type 2 and type 3 ANOVA and are listed in the results section of the main text. Each dot corresponds to a treatment group and its corresponded independent replicate line. N = 6 samples per chromosome and N = 3 samples per group for treatments in all graphs.