Figure 2.

JQ1+ does not reduce proliferation of AtT20 cells after 24 h but does decrease calcium-sensing receptor (CaSR) protein expression. (A) Relative proliferation of AtT20 cells following exposure to DMSO, JQ1− and JQ1+ for 24 and 96 h. Data were expressed relative to cell numbers at day 0. (B) Representative Western blot of CaSR in AtT20 cells following exposure to DMSO, JQ1− and JQ1+ for 24 h. (C) Densitometry analysis of protein expression following treatment with DMSO, JQ1− and JQ1+ for 24 h. CaSR protein expression was expressed relative to calnexin. Data are expressed as mean ± s.e.m. in panels A and C. Statistical analyses compared to DMSO treatment in panel A, ***P < 0.001, and statistical analysis compared to DMSO-treated cells in panel C, **P < 0.005.