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. 2021 Jul 5;67(3):83–94. doi: 10.1530/JME-21-0030

Figure 5.

Figure 5

NPS-2143 has no effect on AtT20 proliferation but does reduce calcium-sensing receptor (CaSR)-mediated cAMP and Ca2+i signalling in AtT20 cells. (A) Effect of NPS-2143 (0–1000 nM) on proliferation of AtT20 cells 2 days and 7 days post-treatment. Data were expressed relative to proliferation at day 1. Statistical analyses comparing proliferation between day 2 and day 7 in each group. There was no significant difference between cell proliferation in cells exposed to different concentrations of NPS-2143. Raw fluorescence units ranged between 17,096 and 69,941 for day 2 and 19,935 and 222,377 for day 7. (B) cAMP-response element (CRE) luciferase reporter responses at 5 mM Ca2+e in AtT20 cells exposed to DMSO or 20 nM NPS-2143 for 12 h. Data were expressed relative to responses in DMSO-treated cells. (C) Ca2+e-induced Fluo-4 intracellular calcium mobilisation assays in AtT20 cells following exposure to DMSO or 20 nM NPS-2143. Raw fluorescence units ranged between 1896 and 58,337. (D) Area under the curve (AUC) of data in C, (E) Maximal Ca2+i responses, and (F) EC50 values showing mean ± 95% CIs obtained in Fluo-4 intracellular calcium mobilisation assays shown in panel C. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05.