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. 2021 Jul 1;12(7):e00377. doi: 10.14309/ctg.0000000000000377

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© 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 3. — Effect of PFKFB3 on proliferation of gastric cancer cells. The mRNA level of PFKFB3 in PFKFB3-overexpressing or PFKFB3-specific shRNA gastric cancer cells was detected by quantitative RT-PCR. All data are shown as mean ± SD and *P < 0.05; ***P < 0.001 (a and b). The cell growth rate was measured by MTT assay with OD490 nm in gastric cancer cells (PFKFB3-overexpressing or PFKFB3-specific shRNA). All data are shown as mean ± SD and **P < 0.01; ***P < 0.001 (c and d). The colony formation assay in gastric cancer cells (PFKFB3-overexpressing or PFKFB3-specific shRNA) (e and f). All data are shown as mean ± SD and **P < 0.01; ***P < 0.001 (g and h). RT-PCR, reverse transcription polymerase chain reaction.