Figure 7.

Combined application of PFK15 could significantly promote the inhibition of tumor progress and metastasis by DDP in nude mice. Real-time RT-PCR analyses revealed DDP/PPK15 combined treatment significantly promoted the expression of E-cadherin and inhibited N-cadherin, Vimentin, Snail, Twist, NF-kB p65, MMP9, and HIF-1α in nude tumors. All the data were shown as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001 (a). A schematic model of PFKFB3 promotes GC growth and metastasis through NF-κB–mediated EMT process, and the application of a PFKFB3 inhibitor could further enhance the therapeutic effect of DDP chemotherapy on GC (b). GC, gastric cancer; RT-PCR, reverse transcription polymerase chain reaction.