Table 1.

Correlation between relative PFKEB3 expression level and clinicopathologic parameters of the patients

Variables	N	PFKFB3 protein level		χ2	P
		Low/normal, n (%)	High, n (%)
Gender
Male	219	71	148	0.064	0.801
Female	100	31	69
Age at diagnosis
<60	165	59	106	2.248	0.134
≥60	154	43	111
Tumor site
Body/antrum	231	77	154	0.71	0.399
Cardia	88	25	63
Lauren classification
Intestinal	293	95	198	0.334	0.564
Diffuse	26	7	19
Helicobacter pylori
Negative	62	26	36	3.51	0.061
Positive	257	76	181
Tumor size
<5 cm	156	82	74	59.501	0.000
≥5 cm	163	20	143
Differentiation
High/medium	143	55	88	5.014	0.025
Low	176	47	129
Venous invasion
No	146	63	83	15.459	0.000
Yes	173	39	134
Nerve invasion
No	126	58	68	18.919	0.014
Yes	193	44	149
Lymphatic invasion
No	73	52	21	67.704	0.000
Yes	246	50	196
Local invasion
No	159	74	85	30.922	0.000
Yes	160	28	132
Invasive depth
T1/T2	49	41	8	71.138	0.000
T3/T4	270	61	209
Nodal metastasis
No	72	51	21	64.553	0.000
Yes	247	51	196
Distant metastasis
No	303	102	201	7.918	0.005
Yes	16	0	16
TNM stage
I/II	119	85	34	135.832	0.000
III/IV	200	17	183

The χ² test was used for statistical analyses. P < 0.05 is considered statistically significant.

PFKFB3 protein high expression is defined as immunohistochemistry score ≥9.

TNM, tumor-node-metastasis.