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SDS-PAGE analysis of recombinant hydrophobin DewA purified by ATPS using IPA. Lane 1, protein size marker; 2, total fraction; 3, soluble fraction; 4, final white precipitate solubilized in 1 mL of 200 mM Tris-HCl buffer (pH 8.0) buffer following purification; 5–8, samples obtained by sequential dilution of previous sample (lane 4) with 1 mL of the same buffer. The arrow indicates the purified RT5-DewA(ΔSS1-24) protein.
