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. 2021 Jul 22;22(15):7843. doi: 10.3390/ijms22157843

Table 2.

Nucleotide sequences of primers and DewA with or without the signal peptide.

Name Nucleotide Sequence
DewA NdeI forward primer ATCATATGCTTCACAGTCCTAATCCCAAGAACGCGAAGCTGGCC
DewA XhoI reverse primer ATACTCGAGTTAGTGGTGGTGGTGGTGGTGCTCAGCCTTGGTACCGGCG
RT5-DewA CTTCACAGTCCTAATCCCCGCTTCATCGTCTCTCTCCTCGCCTTCACTGCCGCGGCCACCGCAACCGCCCTCCCGGCCTCTGCCGCAAAGAACGCGAAGCTGGCCACCTCGGCGGCCTTCGCCAAGCAGGCTGAAGGCACCACCTGCAATGTCGGCTCGATCGCTTGCTGCAACTCCCCCGCTGAGACCAACAACGACAGTCTGTTGAGCGGTCTGCTCGGTGCTGGCCTTCTCAACGGGCTCTCGGGCAACACTGGCAGCGCCTGCGCCAAGGCGAGCTTGATTGACCAGCTGGGTCTGCTCGCTCTCGTCGACCACACTGAGGAAGGCCCCGTCTGCAAGAACATCGTCGCTTGCTGCCCTGAGGGAACCACCAACTGTGTTGCCGTCGACAACGCTGGCGCCGGTACCAAGGCTGAG
RT5-DewA (ΔSS1-24) CTTCACAGTCCTAATCCCAAGAACGCGAAGCTGGCCACCTCGGCGGCCTTCGCCAAGCAGGCTGAAGGCACCACCTGCAATGTCGGCTCGATCGCTTGCTGCAACTCCCCCGCTGAGACCAACAACGACAGTCTGTTGAGCGGTCTGCTCGGTGCTGGCCTTCTCAACGGGCTCTCGGGCAACACTGGCAGCGCCTGCGCCAAGGCGAGCTTGATTGACCAGCTGGGTCTGCTCGCTCTCGTCGACCACACTGAGGAAGGCCCCGTCTGCAAGAACATCGTCGCTTGCTGCCCTGAGGGAACCACCAACTGTGTTGCCGTCGACAACGCTGGCGCCGGTACCAAGGCTGAG

Start codon is intentionally excluded, the RT5 sequence is shaded in yellow, and the signal sequence is shaded in green. Restriction enzyme sites are highlighted in grey.