Table 3.
Sequences of primers and fluorescent probes that were used in the experiments.
| Gene Name | Accession no. for mRNA Sequence | Primer Sequence | Probe Sequence | Product Length |
|---|---|---|---|---|
| KL | NM_004795.4 | F: GCTCAACTCCCCCAGTCAGG R: TGTGGGCTTTGAGAGCTTCG |
CCAGGGCA | 193 |
| FGFR1 | NM_001174063.2 | F: CTTAGGCAAACCCCTGGGAG R: ACAAGGGACCATCCTGCG |
CTGCTGGG | 236 |
| FGFR2 | NM_000141.5 | F: CCGTGAAGATGTTGAAAGATGATGC R: GGTATTCTCGGAGGTTGCCT |
CTTGGAGC | 183 |
| FGFR3 | NM_000142.5 | F: TGCTGAAAGACGATGCCACTG R: CTTGCAGGTGTCGAAGGAGT |
ACCTGCTG | 224 |
| FGFR4 | NM_001291980.2 | F: TTGCCAGCTTCCTACCTGAG R: GCTGGAGGTCAAGGAGTCAC |
CTCTGCCT | 110 |
| ACTB | NM_001101.5 | F: ATTGGCAATGAGCGGTTC R: GGATGCCACAGGACTCCA |
CTTCCAGC | 76 |
ACTB, gene that encodes β-actin; F, forward primer; FGFR1-4, genes that encode fibroblast growth factor receptors 1-4; KL, gene that encodes α-Klotho; R, reverse primer.