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. 2021 Jul 21;22(15):7782. doi: 10.3390/ijms22157782

Figure 5.

Figure 5

CHK1 is required for the transcriptional regulation of Fanconi anemia core protein and FANCD2. (A) U2OS cells were transfected with siControl or siCHK1 and treated with 1 μM mitomycin C (MMC) or 10 μM MG132 for 24 h. Protein expressions were determined from whole cell lysates using antibodies against FANCD2, FANCA, FANCG, FANCL, and CHK1. The asterisk indicates a cross-reactive artifact. (B) After depletion of CHK1, lysates prepared from GM6914/FANCA cell lines were subjected to immunoblot analysis with HA, FANCA, and CHK1 antibodies. (C) After depletion of CHK1, relative mRNA levels of core FA genes, FANCD2, and CHK1 were assessed through RT-qPCR assay in U2OS cells. Data represent the mean ± SD from three independent experiments (*** p < 0.001; ** p < 0.01; * p < 0.05; ns, not significant (p > 0.05)).