Figure 10.

Ca²⁺ signals in control A-172 cells (a) and in response to application of 0.5 µg/mL (b) and 2.5 µg/mL (c) SeNP. BD = application of bi-distilled water equivalent to the amount of SeNP solvent. At the end of the experiment, the Ca²⁺ ionophore, Ionomycin (Iono, 1 µM), or the purinoreceptor activator, ATP (10 µM), was applied to normalize the [Ca²⁺]i signal to the maximum. Typical Ca²⁺ signals of cells are presented.