Figure 14.

Suppression of Ca²⁺ signals in cancer cells in response to application of SeNP after depletion of the Ca²⁺ ER pool by adding the SERCA inhibitor thapsigargin (TG, 1 µM) in a nominally calcium free medium supplemented with 0.5 mM Ca²⁺ chelator EGTA. (a,b) application of 0.5 µg/mL SeNP to A-172 and DU-145 cells; (c,d) application of 10 µg/mL SeNP to MCF-7 and Caco-2 cells. The absence of Ca²⁺ signals for the application of 10 μM ATP indicates a complete absence of Ca²⁺ entering the outside of the cell and mobilization from the ER. Ca²⁺ signals in the field of view of the microscope in one experiment and their average values (gray curves) are presented here.