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. 2021 Jul 22;22(15):7841. doi: 10.3390/ijms22157841

Figure 7.

Figure 7

Losartan altered macrophage polarization in EWAT. (A) Quantification of IL-1β, MCP1, TGFβ, TNFα, and IFNγ by qRT-PCR. qRT-PCR indicates quantification relative to GAPDH. (B) Representative F4/80, CD11b, CD11c, CCR7, CD206 and CD163 staining of EWAT. Red arrow highlights the positive staining. Scale bar: 100 μm. (C) Quantification of CD11c, CCR7, CD206, and CD163 protein levels by Western blot of EWAT. Below graphs indicate quantification relative to β-actin. The ratio of CD11c at CD206 and CCR7 at CD163 in the EWAT. For each animal group, n = 5. All values represent the mean ± SEM. Data were analyzed by Student’s t test. * p ≤ 0.05; normal vs. ob/ob. # p ≤ 0.05; ob/ob vs. ob/ob + Losartan. EWAT, epididymal white adipose tissue; IL-1β, interleukin-1β; MCP1, monocyte chemoattractant protein-1 (CCL2); TGFβ, transforming growth factor beta; TNFα, tumor necrosis factor α; IFNγ, Interferon gamma.