Table 1.
Oligonucleotide sequences for t-qPCR.
| Gene | Forward | Reverse |
|---|---|---|
| ATGL | 5′ aacaccagcatccagttcaa 3′ | 5′ ggttcagtaggccattcctc 3′ |
| HSL | 5′ agacaccagccaacggatac 3′ | 5′ catcaccctcgaagaagagca 3′ |
| LPL | 5′ actcatctccgccatgcc 3′ | 5′ ccagctttctcctagcaagg 3′ |
| ACO | 5′ atgaatcccgatctgcgcaaggagc 3′ | 5′ aaaggcatgtaacccgtagcactcc 3′ |
| SREBP-1c | 5′ actgtcttggttgttgatgagctggagcat 3′ | 5′ atcggcgcggaagctgtcggggtagcgtc 3′ |
| FAS | 5′ tgtcattggcctcctcaaaaagggcgtcca 3′ | 5′ tcaccactgtgggctctgcagagaagcgag 3′ |
| SCD-1 | 5′ ccggagaccccttagatcga 3′ | 5′ tagcctgtaaaagatttctgcaaacc 3′ |
| FATP | 5′ gcttcaacagccgtatcctc 3′ | 5′ tcttcttgttggtggcactg 3′ |
| CD36 | 5′ gcaaaacgactgcaggtcaac 3′ | 5′ tggtcccagtctcatttagcca 3′ |
| CPT-1 | 5′ ggacagagactgtgcgttcct 3′ | 5′ gcgatatccaacagtgcttga 3′ |
| CPT-2 | 5′ caaggccctggctgatgatgtg 3′ | 5′ agtctctgtccgcccctctcg 3′ |
| LCAD | 5′ tcaacagcagttacttgg 3′ | 5′ gacaatatctgagtggag 3′ |
| MCAD | 5′ ggggaggatgacggagcagc 3′ | 5′ cgggtactttaggatctggg 3′ |
| PGC1α | 5′ gactcagtgtcaccaccgaaa-3′ | 5′ tgaacgagagcgcatcctt 3′ |
| TFAM | 5′ ggaatgtggagcgtgctaaaa 3′ | 5′-tgctggaaaaacacttcggaata 3′ |
| UCP1 | 5′ cctgcctctctcggaaacaa 3′ | 5′-tgtaggctgcccaatgaaca 3′ |
| UCP2 | 5′ gcctctggaaagggacttctc 3′ | 5′ accagctcagcacagttgaca 3′ |
| NRF1 | 5′ cgcagcacctttggagaa 3′ | 5′-cccgacctgtggaatacttg-3′ |
| NRF2 | 5′ atggatttgattgacatcctt 3′ | 5′ catgtttttctttgtatctgg 3′ |
| IL-1β | 5′ aacctgctggtgtgtgacgttc 3′ | 5′ cagcacgaggcttttttgttgt 3′ |
| MCP1 | 5′ aggtccctgtcatgcttctg 3′ | 5′ tctggacccattccttcttg 3′ |
| TGFβ | 5′ tatagcaacaattcctggcg 3′ | 5′ tgctgtcacaggagcagtg 3′ |
| TNFα | 5′ ttgacctcagcgctgagttg 3′ | 5′ cctgtagcccacgtcgtagc 3′ |
| IFNγ | 5′ cctcaaacttggcaatactc 3′ | 5′ agcaacaacataagcgtcat 3′ |
| GAPDH | 5′ tcaccaccatggagaaggc 3′ | 5′ gctaagcagttggtggtgca 3′ |
ATGL, adipose triglyceride lipase; HSL, hormone-sensitive lipase; LPL, lipoprotein lipase; ACO, acyl-CoA oxidase; SREBP-1c, sterol regulatory element-binding protein 1c; FAS, fatty acid synthase, SCD-1, stearoyl-CoA desaturase-1, FATP, fatty acid transport protein; CD36, cluster of differentiation 36; CPT-1, carnitine palmitoyltransferase 1; LCAD, long-chain acyl-CoA dehydrogenase; MCAD, medium-chain acyl-CoA dehydrogenase (ACADM); PGC1α, peroxisome proliferator-activated receptor gamma coactivator 1-alpha; TFAM, mitochondrial transcription factor A; UCP1, uncoupling protein 1; NRF1, nuclear respiratory factor 1; IL-1β, interleukin-1β; MCP1, monocyte chemoattractant protein-1 (CCL2); TGFβ, transforming growth factor beta; TNFα, tumor necrosis factor α; IFNγ, interferon gamma; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.