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. 2021 Jul 21;22(15):7766. doi: 10.3390/ijms22157766

Figure 1.

Figure 1

Phylogenetic tree of carbonic anhydrase genes in Lotus and Arabidopsis. The protein sequences were obtained from TAIR (http://www.arabidopsis.org/) (accessed on 19 September 2018) and Kazusa DNA Research Institute (http://www.kazusa.or.jp/lotus/) (accessed on 19 September 2018). Sequence alignment was performed using Clustal W, and the phylogenetic tree was generated by MEGA 7.0 with 1000 bootstrap replications. Blue circles indicate Arabidopsis CA proteins. Red circles indicate Lotus CA proteins. Red stars indicate three LjCAs with enhanced expression in root nodules. The scale bar indicates an evolutionary distance of 0.2 amino acid substitutions per position.