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. 2021 Jul 21;22(15):7766. doi: 10.3390/ijms22157766

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histochemical analysis of GUS expressions driven by LjαCA2 and LjαCA6 promoters in developing root nodules. Expression patterns of GUS reporter were analyzed for pαCA2::GUS (A–D) and pαCA6::GUS (E–H). Nodules at different developmental stages were investigated, including young nodules at 1 wpi (A,E) and mature nodules at 3 wpi (B,C,F,G) and 5 wpi (D,H). pαCA2::GUS and pαCA6::GUS constructs were introduced into wild-type plants using stable transformation. T₂ generation plants were used for GUS staining experiments. Images are representative of at least eight independent transgenic plants. Black arrows indicate the vascular bundle. p, primordia; s, small nodule. Scale bars, 200 μm (A,E); 1 mm (B,F); 100 μm (C,D,G,H).