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. 2021 Jul 21;22(15):7790. doi: 10.3390/ijms22157790

Figure 3.

Figure 3

Effects of HDAC and BET inhibitors on TrkC expression in human neuroblastoma cells. (A–D) Cells were incubated for 24 h with either vehicle, 1 mM VPA, 1 µM entinostat, 20 nM romidepsin or 200 nM vorinostat. Cell lysates were analyzed for TrkC expression by Western blot. Values are the mean ± SD of four independent experiments. * p < 0.05 *** p < 0.001 vs control (vehicle). (E) SH-SY5Y cells were treated for 24 h with either vehicle, 5 µM tubacin, 5 µM PCI-34051 or 1 mM VPA. Cell lysates were analyzed for TrkC, actin, acetyl-Lys9/Lys14 histone H3 (Ac-H3), H3, acetyl-tubulin (Ac-tubulin) and tubulin. Densitometric ratios are reported as fold increase with respect to control (vehicle) and are the mean ± SD of four independent experiments. *** p < 0.001 vs the corresponding control (vehicle). (F) SH-SY5Y cells were incubated for 24 h with the indicated concentrations of VPA. Cell lysates were analyzed for TrkC expression and histone H3 acetylation. Values are the mean ± SD of four independent experiments. (G) SH-SY5Y cells were preincubated for 1 h with either vehicle, 250 nM or 500 nM (+)-JQ1 (JQ1) and then treated with either vehicle or 1 mM VPA for 24 h. Cell lysates were analyzed for TrkC expression. Values are the mean ± SD of four independent experiments. *** p < 0.001 vs. control (vehicle + vehicle); ### p < 0.001 vs. vehicle + VPA by ANOVA followed by Tukey’s test.