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. 2021 Jul 21;22(15):7791. doi: 10.3390/ijms22157791

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Exposure of oxLDL on co-cultured macrophages and endothelial cells. (A) C1q concentration in the supernatant collected from co-culture media was measured using ELISA. (B) The protein levels of C1q in HUVECs were assessed by western blotting in whole-cell lysates at several time points after oxLDL exposure. (C) The protein levels of cleaved IL-1β were analyzed by western blotting in THP-1 whole-cell lysates at several time points after oxLDL exposure. (D) The accumulated lipids in HUVEC (left) and THP-1 (right) cells were stained with BODIPY and were detected by fluorescence microscopy. ***, p < 0.0001.