Figure 3.

Autophagic dysfunction in HUVECs by downregulated p62. (A) Under atherosclerotic conditions derived by oxLDL exposure, the mRNA levels of p62 and LAMP2 were measured by real-time quantitative PCR. (B) After treatment with si-p62 for 24 h, cells were subsequently exposed to oxLDL for 24 h. The protein levels of each molecule were analyzed by western blotting (left), then quantified (right). (C) The consequences of downregulating p62 were evaluated with BODIPY staining for intracellular lipid and immunocytochemistry for p62. (D) Double-immunocytochemistry of LC3 and LAMP2 was done in si-p62 treated HUVECs under oxLDL exposure. ns, no significance; *, p < 0.05; **, p < 0.001, ***, p < 0.0001.