. 2021 Jul 22;22(15):7849. doi: 10.3390/ijms22157849

Table 3.

Bacterial strains and plasmids used in this study.

Strain or Plasmid	Genotypes and Relevant Characteristics ^a	Reference or Source
Strains
E. coli DH5α	F⁻ supE44 ΔlacU169 (ϕ80dlacZψΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Host strain for plasmid cloning and propagation	Invitrogen
E. coli XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacI^qZΔM15 Tn10 (Tet^r)]. Host strain for two-plasmid system	Stratagene
E. coli BW25113/pIJ790	Δ(araD-araB)567 ΔlacZ4787(::rrnB-4) lacIp-4000 (lacI^Q) $\bar{λ}$ rpoS369(Am) rph-1 Δ(rhaD-rhaB) hsdR514. Host strain containing pIJ790 for REDIRECT strategy	[41]
E. coli ET12567/pUZ8002	dam-13::Tn9 dcm-6 hsdM. A methylation-deficient host strain containing non-transmissible pUZ80023 for conjugation	[50]
S. coelicolor M145	wild-type, prototrophic SCP1⁻ SCP2⁻ Pgl⁺	[50]
S. coelicolor ΔsigB	ΔsigB::aac3(IV), Apr^R, deleted sigB gene in S. coelicolor M145 and replaced with the aac3(IV) gene from pIJ773	this study
S. coelicolor ΔsigBop	Δ(rsbB rsbA sigB)::aac3(IV), Apr^R, deleted rsbB, rsbA, sigB operon in S. coelicolor M145 and replaced with the aac3(IV) gene from pIJ773	this study
S. coelicolor ΔsigHop	Δ(ushY ushX sigH)::aac3(IV), Apr^R, deleted ushY, ushX, sigH operon in S. coelicolor M145 and replaced with the aac3(IV) gene from pHP45 aac	this study
Plasmids
pBluescript II SK	Amp^R, standard E. coli cloning vector	Stratagene
LITMUS 28	Amp^R, standard E. coli cloning vector	New England Biolabs
pHP45	Amp^R, standard E. coli cloning vector	[51]
pAPH3	Amp^R, Kan^R, LITMUS 28 derivative containing the neo gene of Tn5	[52]
pHP45 aac	Amp^R, Apr^R, pHP45 derivative containing the aacC4 gene	[53]
pAAC4A	Amp^R, Apr^R, pBluescript II SK derivative containing the aacC4 gene	this study
pSIGSC1	Amp^R, pBR322 derivative containing the ushY, ushX, sigH operon	[28]
pSigHdel1	Amp^R, Apr^R, pAAC4A derivative containing the sigH upstream region	this study
pSigHdel2	Amp^R, Kan^R, pAPH3 derivative containing the sigH downstream region	this study
pSigHdel3	Amp^R, Kan^R, Apr^R, pAPH3 derivative containing deleted sigH operon and replaced with the aacC4 gene	this study
pIJ773	Amp^R, Apr^R, pBluescript KS derivative containing the aac(3)IV gene and oriT	[39]
Stf55	Amp^R, Kan^R, SuperCos-1 derivative containing the sigH operon	[54]
Stf55DSigBop	Amp^R, Kan^R, Apr^R, Stf55 derivative containing deleted sigH operon and replaced with the aac3(IV) gene	this study
Stf55DSigB	Amp^R, Kan^R, Apr^R, Stf55 derivative containing deleted sigH gene and replaced with the aac3(IV) gene	this study
pAC5mut2	Cm^R, expression plasmid vector with the trc promoter	[35]
pAC5-sigF1	Cm^R, pAC5mut2 containing the sigF gene under the trc promoter	[36]
pAC5-sigG1	Cm^R, pAC5mut2 containing the sigF gene under the trc promoter	[20]
pAC5-sigH1	Cm^R, pAC5mut2 containing the sigF gene under the trc promoter	[38]
pAC5-sigB	Cm^R, pAC5mut2 containing the sigB gene under the trc promoter	this study
pAC5-sigI	Cm^R, pAC5mut2 containing the sigI gene under the trc promoter	this study
pAC5-sigK	Cm^R, pAC5mut2 containing the sigK gene under the trc promoter	this study
pAC5-sigL	Cm^R, pAC5mut2 containing the sigL gene under the trc promoter	this study
pAC5-sigM	Cm^R, pAC5mut2 containing the sigM gene under the trc promoter	this study
pAC5-sigN	Cm^R, pAC5mut2 containing the sigN gene under the trc promoter	this study
pAC5-sigF2	Cm^R, pAC5mut2 containing the N-terminally truncated sigF gene under the trc promoter	this study
pAC5-sigH2	Cm^R, pAC5mut2 containing the sigH gene including longer N-terminal region under the trc promoter	this study
pSB40N	Amp^R, promoter probe plasmid containing a promoterless lacZα gene	[36]

^a Cm^R, chloramphenicol resistance; Amp^R, ampicillin resistance; Apr^R, apramycin resistance; Kan^R, kanamycin resistance.