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. 2021 Jul 23;22(15):7874. doi: 10.3390/ijms22157874

Figure 2.

Figure 2

The LMNA-mutated patients iPSCs characteristics. (A) Father, son and control iPSCs showing normal karyotype. (B) Pluripotency staining of LMNA-mutated and control iPSCs. Immunofluorescence staining of typical pluripotent markers Tra1-81 (red), Tra1-60 (red), Oct4 (red), SSEA4 (green) and Nanog (green) in iPSCs generated from father, son and control. Nuclei are stained with DAPI (blue). Scale bar represents 100 µm. (C) Teratoma histological analysis obtained from in vivo differentiated LMNA-mutated and control iPSCs. Teratomas were obtained from SCID-NOD mice injected with either father, son or control iPSCs which differentiated in vivo into derivatives of all three germ layers: ectoderm, endoderm and mesoderm. n—Neuronal tissue represents ectodermal lineage. e—Endodermal epithelium with prominent mucus-producing cells representing endoderm formation. c—Cartilage as well as the chondrocyte area and muscle representing mesoderm formation. Scale bar represents 100 µm.