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. 2021 Aug 6;7(32):eabj1577. doi: 10.1126/sciadv.abj1577

Fig. 3. D-TZP retains bioactivities of PMB.

Fig. 3

SEAP production from THP1-XBlue-MD2-CD14 TLR4 reporter cells incubated for 24 hours with LPS (10 ng/ml) (A) as a mixture with D-TZP or free PMB at different concentrations (n = 3 independently and identically prepared batches) or (B) precomplexed with D-TZP or free PMB for 1 hour and separated from the complexes. trt, treatment. Numbers in the x axis indicate PMB concentration in micrograms per milliliter (n = 3 independently and identically prepared batches for each test sample; n = 6 replicates for LPS control and no-challenge control). Statistical significance was accessed by Dunnett’s multiple comparisons test following ordinary one-way ANOVA (****P < 0.0001; ns, no significant difference). The data are shown as means ± SD. (C) Time-kill assay of K. pneumoniae NR-41920 treated with free PMB or D-TZP at 2× respective minimum inhibitory concentration (MIC). (D) Scanning electron micrographs of K. pneumoniae NR-41920 treated with D5W, free PMB, or D-TZP (at 5× respective MIC) for 1.5 hours. D-TZP is not visible due to the low concentration.