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Droplet-free digital ELISA based on tyramide signal amplification system illustration. (a) Capture of single-protein molecules on beads and labelling with HRP. (b) Reaction of biotinylated tyramides with HRP and conversion into tyramide radicals to deposit onto the beads with labeled HRP. (c) Binding of streptavidin-labeled fluorescent dyes to biotin on the beads. (d) Digital counting of the beads by flow cytometry. Reproduced from reference [40] with permission
