Fig. 7.

A schematic of the solid-phase PLA protocol (SP-PLA). (A) The samples were incubated with pre-immobilized antibodies on microparticles. (B) The microparticles were washed and incubated with pairs of PLA probes. (C) Upon binding of an antigen and adding connector oligonucleotides, the oligonucleotides on the PLA probes were ligated. This process was followed by enhancement and detection of the ligated products by PCR, with the primers shown in arrows. Reproduced from reference [65] with permission