REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Peroxidase AffiniPure Rabbit Anti-Mouse IgG	Jackson ImmunoResearch	Cat# 315-035-045; RRID: AB_2340066
Rabbit Anti-Monkey IgG (whole molecule)-Peroxidase antibody	Sigma-Aldrich	Cat# A2054; RRID:AB_257967
SBA Clonotyping System-HRP kit	SouthernBiotech	Cat#5300-05; RRID:AB_2796080
anti-SARS-CoV-2 Spike monoclonal neutralizing antibody	GenScript	Cat#A02055; RRID: N/A
Bacterial and virus strains
SARS-CoV-2 virus (hCoV-19/France/ lDF0372/2020 strain)	Lescure et al., 2020	EPI_ISL_410720 (GISAID ID)
SARS-CoV-2 (isolate USA-WA1/2020)	BEI resources	Cat#NR-52281
AAVCOVID19-1 (AC1)	Mass Eye and Ear Gene Therapy Vector Core	GenBank: MW408785
AAVCOVID19-3 (AC3)	Mass Eye and Ear Gene Therapy Vector Core	GenBank MW408786
AAVCOVID19-1 (AC1)	Novartis Gene Therapies	GenBank: MW408785
Biological samples
Plasma from COVID-19 convalescent patients	Massachusetts General Hospital	N/A
WHO International Standard for anti-SARS-CoV-2	NIBSC	NIBSC 20/136
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 Wuhan RBD recombinant protein	Schmidt lab, Ragon Institute	N/A
SARS-CoV-2 Spike Glycoprotein D614G peptide pools	GenScript	Cat#RP30025
AC1 and AC3 transgene peptide pools	Mimotopes	N/A
Polyethylenimine or PEI	Polysciences	Cat#24765-2
Benzonase	EMD Millipore	Cat#1016970010
Critical commercial assays
V-PLEX SARS-CoV-2 Panel 2 Kit	Mesoscale Discovery	Cat#K15383U and K12386U
Monkey IFNg ELISpot PRO kit	Mabtech	Cat#3421M-2APT
Experimental models: Cell lines
HEK293 cell line	ATCC	CRL-1573
HEK293T cell line	ATCC	CRL-3216
HEK293T-ACE2 cell line	Hacohen lab	N/A
Experimental models: Organisms/strains
BALB/c mouse strain (Mus musculus)	Jackson Laboratory	Stock#000651
C57BL/6J mouse strain (Mus musculus)	Jackson Laboratory	Stock#000664
Rhesus macaques (Macaca mulatta)	Sichuan Hengshu Bio-Tech Co., Ltd.	N/A
Cynomolgus macaques (Macaca fascicularis)	Cynologics Ltd	N/A
Oligonucleotides
RBD Fw primer:GTGCAGCCAACCGAG	This paper	N/A
RBD Rv primer:ACACCTCGCCAAATGG	This paper	N/A
RBD TaqMan probe:6FAM- TCTATCGTG CGCTTTC-MGBNFQ	This paper	N/A
sgLeadSARSCoV2-F CGATCTCTTGTAG ATCTGTTCTC	Corman et al., 2020; Wolfel et al., 2020	N/A
E-Sarbeco-R primer ATATTGCAGCAG TACGCACACA	Corman et al., 2020; Wolfel et al., 2020	N/A
E-Sarbeco probe HEX-ACACTAGCCA TCCTTACTGCGCTTCG-BHQ1	Corman et al., 2020; Wolfel et al., 2020	N/A
Recombinant DNA
pAAVCOVID19-1 (pAC1)	GenBank	MW408785
pAAVCOVID19-3 (pAC3)	GenBank	MW408786
pKan2/rh32.33	Vandenberghe lab	N/A
pALD-X80	Aldevron	N/A
psPAX2	Hacohen lab	N/A
pCMV-SARS2-Spike	Hacohen lab	N/A
pCMV-Lenti-Luc	Hacohen lab	N/A
Software and algorithms
GraphPad Prism 8	GraphPad	https://www.graphpad.com/
Other
Seracare SureBlue Reserve TMB Microwell Peroxidase Substrate solution	SeraCare	Cat#53-00-03
Seracare KPL TMB Stop Solution	SeraCare	Cat#50-85-06
Reporter Lysis Buffer	Promega	Cat# E4030
Pierce™ Firefly Signal Enhancer	Thermo Fisher Scientific	Cat#16180
D-luciferin	PerkinElmer	Cat#122799