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. 2021 Jul 1;53(8):1187–1195. doi: 10.1038/s41588-021-00891-2

Fig. 6. Punctuated and localized karyotype alterations are ongoing in mature CRC.

Fig. 6

a, Karyotype heatmap showing 112 cells (45% recovery) derived from an excised fragment of a single CRC gland isolated from a primary tumor biopsy of patient C274. The remaining gland structure was isolated as bulk (core). A total of 33 cells showed CNAs that deviated from the core karyotype. Glands were stained with Syto 11 to support imaging (right) and single-cell isolation. Reciprocal CNA of chromosome 2 and propagation of the loss is indicated (lineage I). Several cells show gross genome-wide karyotype alterations (lineage II). b, As in a. A total of 77 single cells were isolated from a wild-type intestinal crypt of patient C575. Four cells showed de novo CNAs of the healthy diploid karyotype. The insets to the right show the excised fragment, obtained by cutting along the solid white lines shown in the main figure.