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. 2021 Aug 6;12:4744. doi: 10.1038/s41467-021-24776-4

Fig. 1. Spheres containing neuromesodermal progenitors generate neurons and myocytes.

Fig. 1

a Sensorimotor organoid differentiation protocol and end-point analysis times. Spheres patterned in suspension are plated after 1 week and grown under adherent conditions for up to 15 weeks. Scale bars are 40 µm for the first two panels, and 1 mm for the third panel. b SOX2+/TBXT+ neuromesodermal progenitors in a typical sphere, after two days in culture. Scale bar, 70 µm. c Distribution of individual spheres according to the expression of myogenic (TBXT), neuromesodermal (TBXT and SOX2), or neurogenic (SOX2) transcription factors, from three independent biological differentiation replicates of each line (colors indicate replicates). Control lines: 11a, FA0000011. ALS lines: MGH5b, 19f, and FA0000012. d Quantification of the SOX2+/TBXT+ area in whole wells of the same iPSC lines. Bars indicate median and IQR. (n = 5–6 organoid cultures obtained from three independent biological differentiation replicates of each of the same five iPSC lines). e Ectodermal lineage cells, including TUJ1+ neurons after 4 weeks in culture. Scale bar, 120 µm. f Mesodermal lineage cells include sarcomeric α-actinin (SAA)+ myocytes, surrounded by Pax7+ cells. Scale bar, 50 µm. g Whole-well confocal image of organoid cultures at 4 weeks showing SAA+ and TUJ1+ areas. Scale bar, 5 mm.