Fig. 7. Impact of WT or MT caspase-8 expression on tumor immune microenvironment in vivo.

MOC1-CASP8 KO cells engineered for DOX-inducible expression of WT caspase-8 or the D303G caspase-8 mutant were inoculated into the flanks of wild-type C57BL/6 mice. On day 28 (see Supplementary Fig. 8), mice were randomized to receive DOX (in drinking water) or no DOX. Tumors were harvested after 18 days of treatment and analyzed by flow cytometry for lymphoid (A) or myeloid (B) cell populations. In the DOX treatment groups, only tumors demonstrating DOX induction of the FLAG-tagged WT or MT caspase-8 (Supplementary Fig. 7) were analyzed. Error bars indicate SEM. Student’s t tests were performed to compare WT with DOX vs. WT without DOX; D303G with DOX vs. D303G without DOX, and WT with DOX vs. D303G with DOX.