Fig. 10. Biocompatibility evaluation of As/AsxOy@PDA@M NSs.
a H&E staining and immunofluorescence (IF) staining in sections from major organs after different treatments with PBS, NSs, or NSs + 660 nm laser irradiation (irradiation performed only within tumor areas). The nucleus was stained by DAPI (blue), damaged DNA by γH2AX foci (red), and apoptotic cells by apoptosis marker C-CAS3 (green). Scale bars = 1000 μm. b Masson staining of pathological fibrous deposition in main organs (kidney, heart, and liver) from mice treated with PBS, NSs, or NSs + 660 nm laser irradiation (irradiations performed only within tumor areas). Scale bars = 1000 μm. c Blood biochemistry and hematology analysis of Balb/c mice treated with As/AsxOy@PDA@M NSs. d Serum levels IFN-γ, IL-6, and TNF-α in healthy mice at 2 or 24 h post intravenous injection of PBS or As/AsxOy@PDA@M NSs. Error bars = standard deviation (n = 3), n = 3 biologically independent mice. For all statistical analysis, data are presented as mean values ± SEM. Two-sided ANOVAs were performed for all other comparisons. No adjustments were made for multiple comparisons. For the H&E staining, IF staining, and Masson staining, three times each experiment was repeated independently with similar results.