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. 2021 Aug 6;4:945. doi: 10.1038/s42003-021-02458-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Detailed information is shown in the Supplementary Note 1. For all panels, the S0 (red) and S30 (green) mean 0 and 30 kg ha⁻¹ sulfur treatments, respectively; DPA days post-anthesis, CoI content index (free amino acid% × 10⁴). The developing grain samples of three replicate pots from the same grain developing stage and sulfur treatment were pooled together, making it 12 pooled developing grain samples with three technical repeats each; line graph shows the means ± 3 technical repeats with a mix of three biologically independent replicates (or three pots); the dots with red and green show the data distribution of Spitfire under S0 and S30, respectively. a The comparison of the dynamics of free total amino acid CoI between S0 and S30 treatments during grain-filling. b The comparison of the dynamics of free methionine (Met) CoI between S0 and S30 treatments during grain-filling. c The comparison of the dynamics of free asparagine (Asn) CoI between S0 and S30 treatments during grain-filling. d The comparison of the dynamics of free aspartic acid (Asp) CoI between S0 and S30 treatments during grain-filling. e The comparison of the dynamics of free glycine (Gly) CoI between S0 and S30 treatments during grain-filling. f The comparison of the dynamics of free cysteine (Cys) CoI between S0 and S30 treatments during grain-filling.