. 2021 Aug 6;4:945. doi: 10.1038/s42003-021-02458-7

Table 3.

Univariate analysis of variance of the impact of the genotypes (G), sulfur treatments (E) and G×E on gluten components in 2014 field trial and glasshouse experiment.

		Glu/gli ratio	Gliadins%	ω5-gliadins%	ω1,2-gliadins%	ω-gliadins%	α/β-gliadins%	γ-gliadins%
Field trial	G*E	0.39^ns	0.77^ns	4.93**	3.22*	1.10^ns	0.39^ns	0.90^ns
	Genotype	3.25*	5.25**	0.84^ns	17.38***	11.53***	6.77**	5.23**
	Treatment	33.63***	47.66***	81.96***	44.69***	29.39***	27.48***	33.44***
Glasshouse experiment	G*E	6.88*	7.47**	2.52^ns	1.42^ns	3.87*	2.61^ns	6.64*
	Genotype	7.98*	9.93**	0.07^ns	1.77^ns	0.85^ns	0.43^ns	45.45***
	Treatment	26.25***	37.13***	23.86***	17.26***	26.75***	24.38***	18.79***

The SPSS univariate analysis of variance (UNIANOVA) in general linear model was used to calculate the F-values of the four genotypes (G), three sulfur treatments (E) and G×E on each gluten component. *P < 0.05, **P < 0.01, ***P < 0.001; ^nsnot significant; the number of biologically independent replicate (plot or pot) for each genotype under each sulfur treatment is 3. Under a fixed nitrogen application rate of 25 kg ha⁻¹, the statistically significant impacts of sulfur treatments on the ratio of glutenin to gliadin (glu/gli ratio), the percentages of gliadins (gliadins%) and its each subtype (ω-gliadins%, α/β-gliadins% and γ-gliadins%) in the total gluten content were observed in both 2014 field trial and glasshouse experiment.