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. Author manuscript; available in PMC: 2021 Aug 27.
Published in final edited form as: Hepatology. 2021 Aug 10;74(2):667–685. doi: 10.1002/hep.31743

Figure 4: Heterogeneity of quiescent HSCs in murine NASH.

Figure 4:

a) Previously determined markers of central-vein associated qHSCs (yellow) and portal-vein associated qHSCs (green) correspond to clusters Q0 and Q2 (quiescent clusters) respectively. Heatmap displays relative gene expression in cells binned by UMAP dimension 1, where most of the variance is seen between clusters Q0 and Q2, bins evenly spaced between minimum and maximum UMAP-1 value for cells in clusters Q0 and Q2. Cluster membership annotated by top color bar and dotted line. Inset indicates breakdown of cells in clusters 0 and 2 by sample. b) Individual cell expression plotted in UMAP space for cells in clusters Q0 and Q2, for four selected marker genes of portal-vein HSCs (Ngfr, Itgb3), and central-vein associated HSCs (Adamtsl2, Rspo3). c) Markers of cluster Q2 identified by our analysis overlap with portal-vein markers (33/46 portal-vein genes shared) d) Markers of cluster Q0 identified by our analysis overlap with central vein marker genes (20/26 central-vein genes shared). e) Molecular interactions among cluster Q2 marker genes, from the STRING database. This gene set has more interactions than expected by chance (p<1E-16, permutation test). f) Molecular interactions among cluster Q0 marker genes, from the STRING database. This gene set has more interactions than expected by chance (p<1E-16, permutation test).