Figure 3.

Effect of Dex on metabolic phenotype in the offspring

(A) Experimental design depicting timeline between injection, sperm harvest, in vitro fertilization, and phenotyping.

(B–D) (B) Impact of Dex on male and female adult offspring. (B) Body mass index (males vehicle offspring n = 21, Dex offspring n = 22, females vehicle offspring n = 17, Dex offspring n = 17), (C) glucose tolerance (males vehicle offspring n = 12, Dex offspring n = 12, females vehicle offspring n = 12, Dex offspring n = 12), and (D) insulin tolerance (males vehicle offspring n = 9, Dex offspring n = 8, females vehicle offspring n = 12, Dex offspring n = 12). Error bars display SEM. Detailed statistical results are depicted in Figure S5, and raw data are provided in Table S3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, 3-way repeated-measures ANOVA.