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. 2021 Jul 16;24(8):102870. doi: 10.1016/j.isci.2021.102870

Figure 6.

Figure 6

Effects of Dex on long RNA payload of sperm cells residing in testes at the time of administration

(A) Experimental design depicting a time window of two weeks between injection of Dex and sperm collection for molecular analysis.

(B) Volcano plot depicting fold changes and significance level of long RNA in mature sperm 14 days after injection of Dex (n = 4) versus vehicle (n = 4) as assessed by small RNA sequencing. Statistically significant transcripts are highlighted in red (FDR <0.05).

(C) Heatmap showing significantly differentially expressed long RNA transcripts of the same experiment (multiple comparison corrected, q < 0.05).

(D) Q-PCR results of CircRNA assays for caput and cauda sperm for Tasp1 (interaction treatment x tissue F(1,5) = 7.53, p < 0.05, caput: control n = 4, DEX n = 5, p > 0.05; cauda control n = 5, DEX n = 3, p < 0.01) (∗p < 0.05 mixed-effect model interaction [REML], ∗∗q < 0.01 multiple comparisons SIDAK corrected) and Dennd1 (interaction treatment x tissue F(1,15) = 6, p < 0.05, caput: control n = 4, DEX n = 5, p > 0.05; cauda control n = 5, DEX n = 5, p < 0.05) (E). Whiskers display minimum and maximum. ∗p < 0.05 mixed-effect model interaction (REML), ∗q < 0.05 multiple comparisons SIDAK corrected.