Figure 3.

Inulin-enriched diet prevented STZ-induced kidney and pancreatic islet damage. (A–C) STZ-treated and untreated mice were euthanized at end of experiment (12 weeks and STZ); kidney weight (A) and images of kidney in STZ-treated mice (B); blood urea nitrogen (BUN) in serum of mice measured by enzyme-linked immunosorbent assay (C). (D–H) Pancreas weight (D) and insulin staining per section were calculated using ImageJ software (E); representative image of HE and immunofluorescence microscopy; insulin (green), counterstained with DAPI (blue) in STZ-treated mice (F). (G and H) MCP-1 (G) and IL6 (H) in pancreas measured by quantitative real-time PCR. Data are means ± standard error of the mean (n = 4–9 mice per group) of an individual experiment and representative of at least 2 separate experiments.