Figure 9.

IL22 was necessary for inulin’s prevention of STZ-induced diabetes. (A) Mice (n = 4–5), WT and IL22 KO, were fed CDD or CDD:Inul diets and treated with STZ to induce hyperglycemia. Expression of IL22 was analyzed by using ex vivo culture of colonic tissue of WT mice. (B) Blood glucose was monitored. (C and D) Epididymal fat (C) and the pancreas (D) were weighed at end of experiment. (E–G) Insulin sensitivity check index was calculated (E); insulin tolerance test (ITT) was conducted as mentioned (F); area under curve of ITT was calculated (G). (H) Kidney weight. (I) Feces was collected to measure SCFA. (J) Expression of IL22 was analyzed by using ex vivo culture of colonic tissue of WT mice fed with indicated diets with or without β-acid treatment.