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. 2021 May 7;12(3):1001–1019. doi: 10.1016/j.jcmgh.2021.04.013

Figure 2.

Figure 2

HFCFr diet feeding for 42 weeks results in liver tumor development in mice. (A) Representative image of NASH liver and NASH-tumor liver. (B) Number of tumor-bearing mice in the 42-week HFCFr diet cohort. (C) Representative H&E staining. (D) Representative Sirius Red staining. (E) Representative reticulin stain of liver sections showing tumor and surrounding non-tumor tissue. (Scale bar = 250 μm). (F) Mean body weight. (G) Liver weight. (H) NAS and fibrosis score of non-tumor tissues of tumor-bearing livers. All results are mean ± SEM. Student t test was used to calculate the P value for F and G. ∗ vs non–tumor-bearing mice. Tumor-bearing mice (n = 4) and non–tumor-bearing mice (n = 21).