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. 2021 May 28;12(3):873–889. doi: 10.1016/j.jcmgh.2021.05.014

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Esrrg abolition by genomic RNA (gRNA) 2 impairs Sox8 activation and the functional maturation of M cells. (A) Esrrg protein expression in Esrrg KO2 with gRNA 2 and Scrambled cells generated by lentiCRISPR v2 genome editing in C57BL/6JRj intestinal organoids. Organoid lysates were analyzed by Western blot. (B) qPCR analysis of M-cell–associated genes expressed in Esrrg KO2 and Scrambled intestinal organoids stimulated by RankL for 4 days. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. Values in all are presented as the means ± SD. Unpaired 2-tailed Student t test, N = 3 independent experiments ∗P < .05, ∗∗P < .01.