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. 2021 May 28;12(3):873–889. doi: 10.1016/j.jcmgh.2021.05.014

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Esrrg expression and its relation to other M-cell developmental markers Spi-B and Sox8. (A) Esrrg expression was unaffected by lack of Sox8 expression. qPCR analysis of Esrrg and Gp2 in the FAE and villous epithelium from Sox8+/+ and Sox8-/- mice. (B) Organoids generated from Sox8+/+ and Sox8-/- mice were stimulated with and without RankL for 4 days. Esrrg expression was examined by qPCR analysis. (C) Organoids isolated from Sox8 wild-type and Sox8 KO mice were lysed and analyzed by Western blot for Esrrg expression. (D) Spi-B protein expression in Spi-B KO and Scrambled cells generated by lentiCRISPR v2 genome editing in C57BL/6JRj intestinal organoids. Organoid lysates were analyzed by Western blot. (E) qPCR analysis of Esrrg in a Spi-B KO intestinal organoid by lentiCRISPR v2. (F) Organoid lysates for Esrrg in Scrambled and SpiB KO organoids were analyzed by Western blot. Values in all are presented as the means ± SD. Unpaired 2-tailed Student t test, N = 3. ∗P < .05, ∗∗P < .01. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; rRNA, ribosomal RNA.