Figure 5.

Feeding hormones de-induce AA catabolism gene expression back to basal levels. Primary hepatocytes were treated for 2 hours with gluc, cort, or both in a dual treatment (gluc + cort). Then hepatocytes were washed with PBS and treated with insulin or FGF19 for 1 hour. RNA was isolated, and levels of fasting-induced AA catabolism genes were measured by qPCR (A). Synergistic gene induction, as well as single-treatment gene induction, was rapidly and potently repressed by insulin (B) and FGF19 (C). Graphs represent data collected from 3 independent replicates. Statistical significance was determined by t test; P value was adjusted for multiple comparisons using the Holm-Sidak method. Single asterisk denotes statistical significance of adjusted P value ≤.05. Double asterisks denote statistical significance of adjusted P value ≤.01. ns denotes P value >.05.