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. 2021 Jun 6;12(3):943–981. doi: 10.1016/j.jcmgh.2021.05.019

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Genome-wide gene expression analysis of sorted intestinal epithelial cells. (A) Experimental design of genome-wide gene expression analysis of fluorescence-activated cell sorter (FACS)-sorted P20 intestinal epithelial cells. (B) Percentage of EpCAM stained cells in the total number of FACS-sorted intestinal epithelial cells per condition. (C) Principal component analysis (PCA) of sorted epithelial cells from P20 proximal and distal small intestine treated with the antibiotic mix or PBS (control). (D) Volcano plots of microarray analysis showing genes differentially expressed between control and antibiotic-treated FACS-sorted P20 intestinal epithelial cells. Green dots identify down-regulated genes and red dots identify up-regulated genes. Statistical analysis by empirical Bayes analysis of variance, P < .05 cut-off. n = 4 samples per group.