Figure 7.

Appearance and budding quantification of proximal and distal fetal intestinal organoids treated with early life antibiotics over time. (A) Experimental design of in vitro treatment of mouse fetal intestinal organoids with the antibiotic mix. Organoids were analyzed on days 3, 13, 20, and 27 of culture. (B) Microscopic images of control and antibiotic mix–treated organoids on days 3, 13, 20, and 27 of culture. Scale bars: 500 μm. (C) Quantification of the number of buds per organoid of proximal and distal cultures in control and antibiotic mix conditions. Statistical analysis was performed by a 1-tailed paired t test. Error bars indicate means ± SD. Levels of significance are indicated: ∗P < .05, ∗∗P < .01). n = 18–57 organoids per condition and day of 4 independent cultures.