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. 2021 May 11;12(3):857–871. doi: 10.1016/j.jcmgh.2021.05.006

Figure 2.

Figure 2

Administration of NaB stimulates the activity of Insig to inhibit lipogenic gene expression in mice fed with HFD. (A–C) The protein levels of Insig-1 and Insig-2 are increased in the liver of mice treated with NaB. (A) Representative immunohistochemical staining of the liver sections with Insig-1 and Insig-2 antibodies (scale bar = 50 μm). (B) Immunoblots were performed, and the band intensity of (C) Insig-1 and (D) Insig-2 was quantified by densitometry (n = 6). (E–I) The protein levels of lipogenic genes are decreased in the liver of mice treated with NaB. (E) Representative immunohistochemical staining of the liver sections with SCD1 and FAS antibodies (scale bar = 50 μm). (F) Immunoblots were performed, and the band intensity of (G) SCD1, (H) FAS, and (I) nuclear SREBP-1 was quantified by densitometry (n = 6). The data are presented as the mean ± SEM, unpaired 2-tailed Student’s t test, n = 6. ∗P < .05 vs mice fed with chow diet; #P < .05 vs mice fed with HFD.